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| Registros recuperados: 4.144 | |
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| Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto. |
| Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in... |
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Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574 |
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| Abdelbaset, E. Abdelbaset; Fox, Barbara A.; Karram, Mohamed H.; Abd Ellah, Mahmoud R.; Bzik, David J.; Igarashi, Makoto. |
| In the asexual stages, Toxoplasma gondii stage converts between acute phase rapidly replicating tachyzoites and chronic phase slowly dividing bradyzoites. Correspondingly, T. gondii differentially expresses two distinct genes and isoforms of the lactate dehydrogenase enzyme, expressing LDH1 exclusively in the tachyzoite stage and LDH2 preferentially in the bradyzoite stage. LDH catalyzes the interconversion of pyruvate and lactate in anaerobic growth conditions and is utilized for energy supply, however, the precise role of LDH1 and LDH2 in parasite biology in the asexual stages is still unclear. Here, we investigated the biological role of LDH1 and LDH2 in the asexual stages, and the vaccine strain potential of deletion mutants lacking LDH1, LDH2, or both... |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4451 |
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| ABE, Yasuyuki; LEE, Dong-Soo; SANO, Hikaru; AKIYAMA, Koji; YANAGIMOTO-UETA, Yoshiko; ASANO, Tomoyoshi; SUWA, Yoshinori; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Due to the recent outbreak of avian influenza, transportation of frozen canine semen with egg yolk has been sharply restricted. Thus, there is urgent need to develop a novel egg yolk-free extender for freezing canine spermatozoa. In the present study, the effect of using skim milk/glucose (SG)-based extender without egg yolk on the motility and fertilizing capacity of canine spermatozoa frozen-thawed in the presence of glycerol was examined. There was a tendency for the proportion of motile spermatozoa exposed to SG-based extender for 3 h to be higher than that exposed for 1 h, but the difference was not significant. The motility and other viability parameters of canine spermatozoa after thawing were similar to those obtained with an egg yolk-based... |
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Palavras-chave: Canine; Cryopreservation; Skim milk/glucose-based extender; Spermatozoa. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3030 |
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| ABE, Yasuyuki; SUWA, Yoshinori; YANAGIMOTO-UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志. |
| Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further... |
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Palavras-chave: Dog; Embryo; Preimplantation development. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3029 |
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| Abouelnasr, Khaled; Hamed, Mohamed; Lashen, Samah; El-Adl, Mohamed; Rasha, Eltaysh; Tagawa, Michihito. |
| Platelet-rich plasma (PRP) has an important role in musculoskeletal surgery; however, it has been underutilized for accelerating the healing of abdominal wall defects in veterinary practice. Therefore, the aim of this study was to evaluate the use of commercial polyester/cotton fabric (Damour) as a new composite mesh for the repair of experimentally induced abdominal wall defects in canine models, and to investigate the possible role of PRP for improving such repair and reducing allied complications. For this purpose, abdominal wall defects were created in 24 healthy mongrel dogs and then repaired with mesh alone (control group) or mesh and allogenic PRP (PRP group). Dogs were euthanized after 2 or 4 months for gross examination of implantation site,... |
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Palavras-chave: Abdominal wall defect; Damour; Dog; Platelet-rich plasma. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4542 |
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| AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男. |
| This study of enteric parasites of ducks was undertaken to determine their prevalence and the relationships between their prevalence and the age and breed of ducks in the Behera governorate, Egypt. A total of 110 ducks of different ages and breeds were examined. The prevalence of helminths was 4.54% from which the prevalence of nematode, cestode, and trematode infections was 2.72%, 3.63%, and 1.81%, respectively. The recovered nematodes were Ascaridia galli (0.9%) and Heterakis gallinarum (1.81%). The reported trematodes were Echinoparyphium recurvatum (0.9%) and Echinoparyphium paraulum (1.81%). The new reported cestodes were Cladogynia phoeniconaiadis (3.63%), Echinolepis carioca (3.63%), and Baerfainia anoplocephaloides (3.63%). The helminths infections... |
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Palavras-chave: Ducks; Enteric parasites; Egypt. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3125 |
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| AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男. |
| Toxoplasma gondii is an important zoonotic parasite. The diagnosis of infection in animals is an important tool to prevent human infection. In the present study, Toxoplasma gondii was diagnosed by using the modified agglutination test (MAT) in ducks from Behera Governorate, Egypt. The prevalence of Toxoplasma gondii was 13.9%. The prevalence of Toxoplasma gondii was the highest in the Native breed (17.65%) and in 6-8-months age group (19.4%). End-point titer of 1:25, 1:50, and 1:100 were recorded in 61.9%, 19.05%, and 19.05% of the positive samples, respectively. Duck meat is one source of the Toxoplasma gondii infection for human in Egypt. |
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Palavras-chave: Toxoplasma gondii; Modified agglutination test (MAT); Ducks; Egypt. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3128 |
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| Abubakar, Y.U.; Oyedipe, E.O.; Eduvie, L.O.; Ogwu, D.O.; Adeyeye, A.A.. |
| Ten cycling Nigerian West African Dwarf (WAD) ewes were used to determine the effect of Trypanosoma congolense infection on the estrous cycle. The estrus of ewes were synchronized using intravaginal progesterone sponges and divided into 2 groups (A and B) of 5 ewes each after 54 days (3 cycles). The ewes in group A were inoculated with blood containing approximately 5×105 trypanosomes per ml of blood while ewes in group B served as the uninfected control. All the ewes were monitored from infection till the end of the study (56 days). Ewes in the infected group had silent estrus with clinical trypanosomosis that was characterized by undulating parasitemia, intermittent pyrexia, anemia and weight loss. There was no statistical (p > 0.05) difference... |
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Palavras-chave: Infertility; Estrous cycle; Estrus; Trypanosoma congolense; West African dwarf ewe. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4244 |
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| ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h... |
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Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979 |
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| ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and... |
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Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980 |
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| Adeyemi, Oluyomi Stephen; Murata, Yuho; Sugi, Tatsuki; Han, Yongmei; Kato, Kentaro. |
| Background Toxoplasmosis constitutes a large global burden that is further exacerbated by the shortcomings of available therapeutic options, thus underscoring the urgent need for better anti-Toxoplasma gondii therapy or strategies. Recently, we showed that the anti-parasitic action of inorganic nanoparticles (NPs) could, in part, be due to changes in redox status as well as in the parasite mitochondrial membrane potential. Methods In the present study, we explored the in vitro mode of action of the anti-T. gondii effect of NPs by evaluating the contributions of host cellular processes, including the tryptophan pathway and hypoxia-inducing factor activity. NPs, at concentrations ranging from 0.01 to 200 μg/ml were screened for anti-parasitic activity.... |
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Palavras-chave: Hypoxia Indoleamine 2; 3-dioxygenase Mechanism of action Nanomedicine Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4539 |
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| Adeyemi, Oluyomi Stephen; Murata, Yuho; Sugi, Tatsuki; Kato, Kentaro. |
| This study evaluated the anti-Toxoplasma gondii potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01-1,000 mu g/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of T. gondii growth with EC50 values of <= 7, <= 1, and <= 100 mu g/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti-T. gondii concentrations; the estimated selectivity index revealed a >= 20-fold activity toward the parasite versus the host cell. The anti-T. gondii activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results... |
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Palavras-chave: Antiparasite; Drug screening; Nanomedicine; Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4450 |
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| Adrian Patalinghug Ybañez; Rochelle Haidee Daclan Ybañez; Yokoyama, Naoaki; Inokuma, Hisashi. |
| Aim: Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs. Materials and Methods: DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR). Infection was confirmed by sequencing of positive amplicons. Second round PCR targeting a longer 16S rRNA fragment was subsequently performed on the first round PCR amplicons of the positive samples. Further characterization using the... |
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Palavras-chave: Anaplasma platys; Dogs; Philippines; Variants. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4537 |
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| Registros recuperados: 4.144 | |
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